pET-12a(+)大肠杆菌表达载体说明

1、pET-12a(+)编号载体名称北京华越洋生物 VECT4090pET-12a(+)pET-12a(+)载体基本信息别名:pET-12a, pet 12a质粒类型:大肠杆菌表达载体表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:4674bp5 测序引物:T7/ lacI5 测序引物序列:5-TAATACGACTCACTATAGGG-3载体标签:OmpT载体抗性:Ampicillin克隆菌株:DH5a表达菌株:BL21 系列备注:Hosts: E.coli. Related vectors: pBR322；pET-12a，b，c 仅阅读框（读码框）不同，彼此相差 1 个 bp。稳定性:稳

2、表达组成型:诱导型（IPTG)病毒/非病毒:非病毒pET-12a(+)载体质粒图谱和多克隆位点信息pET-12a(+)载体序列ORIGIN1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT181 GCGGGATATC GTCCATTCCG A

3、CAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC361 CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA421 CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGC

4、GGTGGCA GCAGCCAACT481 CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCCCGTCG ACGCAAAAGA GCTGATCGCG541 ATAGGGGTTG TCAGGACTAT TCCTAGGAGT TTCGCCCGCA TATGTATATC TCCTTCTTAA601 AGTTAAACAA AATTATTTCT AGAGGGAAAC CGTTGTGGTC TCCCTATAGT GAGTCGTATT661 AATTTCGCGG GATCGAGATC TCGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG721 GC

5、GCCACAGG TGCGGTTGCT GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG781 CTCGCCACTT CGGGCTCATG AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG841 CCGGGGGACT GTTGGGCGCC ATCTCCTTGC ATGCACCATT CCTTGCGGCG GCGGTGCTCA901 ACGGCCTCAA CCTACTACTG GGCTGCTTCC TAATGCAGGA GTCGCATAAG GGAGAGCGTC961 GATCGACCGA TGCCCTTGAG AGCC

6、TTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC1021 ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG1081 CCGGCAGCGC TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC1141 GGCCTGTCGC TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT CGTCACTGGT1201 CCCGCCACCA AACGTTTCGG CGAGAAGCAG GCCATTATCG CC

7、GGCATGGC GGCCGACGCG1261 CTGGGCTACG TCTTGCTGGC GTTCGCGACG CGAGGCTGGA TGGCCTTCCC CATTATGATT1321 CTTCTCGCTT CCGGCGGCAT CGGGATGCCC GCGTTGCAGG CCATGCTGTC CAGGCAGGTA1381 GATGACGACC ATCAGGGACA GCTTCAAGGA TCGCTCGCGG CTCTTACCAG CCTAACTTCG1441 ATCACTGGAC CGCTGATCGT CACGGCGATT TATGCCGCCT CGGCGAGCAC ATGGAACGGG1

8、501 TTGGCATGGA TTGTAGGCGC CGCCCTATAC CTTGTCTGCC TCCCCGCGTT GCGTCGCGGT1561 GCATGGAGCC GGGCCACCTC GACCTGAATG GAAGCCGGCG GCACCTCGCT AACGGATTCA1621 CCACTCCAAG AATTGGAGCC AATCAATTCT TGCGGAGAAC TGTGAATGCG CAAACCAACC1681 CTTGGCAGAA CATATCCATC GCGTCCGCCA TCTCCAGCAG CCGCACGCGG CGCATCTCGG1741 GCAGCGTTGG GTCCT

9、GGCCA CGGGTGCGCA TGATCGTGCT CCTGTCGTTG AGGACCCGGC1801 TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC AGAATGAATC ACCGATACGC GAGCGAACGT1861 GAAGCGACTG CTGCTGCAAA ACGTCTGCGA CCTGAGCAAC AACATGAATG GTCTTCGGTT1921 TCCGTGTTTC GTAAAGTCTG GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA1981 TCTGCATCGC AGGATGCTGC TGGCTACCCT GTG

10、GAACACC TACATCTGTA TTAACGAAGC2041 GCTGGCATTG ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG CATCCATACC GCCAGTTGTT2101 TACCCTCACA ACGTTCCAGT AACCGGGCAT GTTCATCATC AGTAACCCGT ATCGTGAGCA2161 TCCTCTCTCG TTTCATCGGT ATCATTACCC CCATGAACAG AAATCCCCCT TACACGGAGG2221 CATCAGTGAC CAAACAGGAA AAAACCGCCC TTAACATGGC CCGCTTTATC A

11、GAAGCCAGA2281 CATTAACGCT TCTGGAGAAA CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG2341 AATCGCTTCA CGACCACGCT GATGAGCTTT ACCGCAGCTG CCTCGCGCGT TTCGGTGATG2401 ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG2461 ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG2521 CAGCCA

12、TGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC2581 AGAGCAGATT GTACTGAGAG TGCACCATAT ATGCGGTGTG AAATACCGCA CAGATGCGTA2641 AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG2701 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA2761 GAATCAGGGG ATAACGCAGG AAAG

13、AACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC2821 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC2881 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG2941 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC3001 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AT

14、AGCTCACG CTGTAGGTAT3061 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG3121 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC3181 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT3241 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT3

15、301 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC3361 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA3421 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC3481 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC3541 CTTTTAAATT AAAAA

16、TGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT3601 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA3661 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT3721 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA3781 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGA

17、AGTGGTC CTGCAACTTT ATCCGCCTCC3841 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG3901 CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT3961 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA4021 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC C

18、GCAGTGTTA4081 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC4141 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG4201 AGTTGCTCTT GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA4261 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG4321 AGATCC

19、AGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC4381 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG4441 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT4501 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA4561 GGGGTTCCGC GCACATTTCC CCGA

20、AAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC4621 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA AGAA/其他大肠杆菌表达载体：pBV221ptdTomatopET-52b(+)pAmCyanpDsRed-Express2pBV220pCold-GSTpColdS-SUMOpColdTFpCold IVpCold IIIpCold IIpColdIpE-SUMOpCold-ProS2pBAD102/D-TOPOpBAD202/D-TOPOpACYC184pBAD/Thio-TOPOpBad/M

21、yc-His CpBad/Myc-His BpBad/Myc-His ApBad/His CpBad/His BpBad/His ApBAD-TOPOpET-23b(+)pET-23a(+)pET-23c(+)pET-23(+)pET-12b(+)pET-12c(+)pET-12a(+)pET-11b(+)pET-11a(+)pET-11c(+)pBad24pQE-82LpQE-81LpQE-80LpQE-32pQE-9pQE-16pQE-31pQE-60pQE-70pQE-40pET-51b(+)pET-50b(+)pET-49b(+)pET-48b(+)pET-47b(+)pET-26b(

22、+)pET-32a(+)pET-21b(+)pET-22b(+)pET-14bpET-16bpET-15bpET-19bpET-20b(+)pET-21d(+)pET-21c(+)pET-21b(+)pET-21a(+)pET-24a(+)pET-24d(+)pET-25b(+)pET-27b(+)pET-28a(+)pET-30a(+)pET-42a(+)pET-43.1c(+)pET-43.1b(+)pET-43.1a(+)pET-44a(+)pET-44c(+)pET-46 EK/LICpET-37b(+)pTrcHis2 CpTrcHis2 BpTrcHis2 ApET303/CT-H

23、ispET302/NT-HispRSET-CFPpRSET-EmGFPpRSET-BFPpGFPuvpET300/NT-DESTpET301/CT-DESTpGEM-TpBad43pGEX-4T-3pGEX-5X-2pBlueScript SK(+)pG-Tf2pG-KJE8pGro7pET-SUMOpSE380pET-17bpET102/D-TOPOpCDFDuet-1pMAL-p5xpTf16pET-28c(+)pBluescript II SK(+)pET-30b(+)pSUMOpProEX HTcpProEX HTbpProEX HTapKD3pKD13pKD46pTYB1pTYB2p

24、TWIN2pBluescript II KS(-)pTYB12pMAL-p5epACYCDuet-1pEGM-11ZF(+)pEGM-7ZF(+)PinPoint Xa-3PinPoint Xa-2PinPoint Xa-1pSP73pSP64pTWIN1pTYB11pTXB1pET-5b(+)pBad/gIII CpBad/gIII BpBad/gIII ApET-5a(+)pMal-p4XpMal-p2Gpkk223-3pkk232-8pCYB1pEZZ18pBAD18pMAL-c5xpMal-p2EpMal-p2XpET-44 EK/LICpET-43.1 EK/LICpET-41 EK

25、/LICpMal-c4XpTrcHis BpET-31b(+)pET-3b(+)pET-41a(+)pGEX-3XpGEX-4T-2pETDuet-1pGEX-4T-1pTrc99apET-28b(+)pET-HispALEX a,b,cpACYC177pBR322pKD4pKD20pMXB10pEcoli-6xHN-GFPuvpKJE7pRSET BpGEX-KGpGEX-2TpRSFDuet-1pCOLADuet-1pTrcHis CpTrcHis ApET-41b(+)pET-42b(+)pET-3a(+)pGEX-6P-3pGEX-6P-2pGEX-6P-1pGEX-5X-3pGEX-5X-1pGEX-2TKpRSET ApMal-c2GpMal-c2EpMal-c2XpRSET CpQE-30pET-45b(+)pET-44b(+)pET-42c(+)pET-41c(+)pET-40b(+) pET-32 Xa/LIC pET-30 EK/LIC pET-29a(+) pET-23d(+)pET-33b(+)pET-39b(+)pET-32EK/LICpET-32c(+)pET-32b(+)pET-30Xa/LICpET-30c(+)pET-29c(+)pET-29b(+)pET-24c(+)pET-24b(+)pET-24(+)pET-11d(+)pBad33